IOWA STATE UNIVERSITY | DEPARTMENT OF AGRONOMY | COLLEGE OF AGRICULTURE AND LIFE SCIENCES | PLANT SCIENCES INSTITUTE

Maize Genetic Transformation:
Biolistic-Mediated

Pricing for 2008-2009


Biolistic-Mediated Maize Transformation Services On Campus Off Campus
Hi II*
Transgenic plantlets (per event**) $146.00 $292.00
Transgenic seed (per event) $236.00 $488.00
Transgenic callus (per bombardment) $250.00 $509.00
Non-standard type II tissue
Transgenic plantlet (per event) $292.00 N/A
Transgenic seed (per event) $382.00 N/A

* A minimum of 10 events per construct can be ordered. Contact Kan Wang for questions, or to place orders.

** Transgenic "event" refers to a transgenic line that has one or more transgenes integrated into the genome of a single cell. Typically multiple events (or lines) are generated for any DNA construct carrying gene of interest. Each transgenic event (or line) represents an independent transgene integration event.


Product Description

Plantlet

Bialaphos resistant plantlets rooted in a petridish containing regeneration medium. Typically, 2-4 plants per event are sent and usually 15 events are sent when 10 events are ordered

Seed

Seed from 2-4 Bialaphos resistant plants per event are sent at the completion of the project. An event producing more than 50 seeds is considered fertile.

Callus

Bialaphos resistant callus cultures. Each bombardment yields about 25 [bar]+ callus events(for Hi II) of which 60-90% will contain your gene of interest (when co-bombarded with the bar construct).