IOWA STATE UNIVERSITY | DEPARTMENT OF AGRONOMY | COLLEGE OF AGRICULTURE AND LIFE SCIENCES | PLANT SCIENCES INSTITUTE

Choose a service

a. What genotype or cultivar do you want to transform?


Maize:
Our pipeline genotype is Hi II, a hybrid (with A188 and B73 background1) amenable for both Agrobacterium- and biolistic-mediated transformation methods. Our inbred genotype is B104, a public inbred derives from BS13(S)C5, an Iowa Stiff Stalk Synthetic (BSSS) population2. It also shares some percentages (~ 60%) of genetic similarity with B733.

Hi II genotype is suitable for most projects involved in gene expression and functional analysis. However, if you are working with gene(s) confers quantitative traits, the segregation of the Hi II hybrid background can complicate the analysis. In this case, using an inbred background may be more desirable.

Hi II B104
Endogenous gene segregation in progeny Yes, traits contributed by parent A188 or B73 will segregate in progeny No, traits should not segregate
Transformation waiting time No Yes, 3 months advance order
Transformation methods Agrobacterium, biolistic Agrobacterium
Cost $304/event (plantlet)
$476/event (seed) 		$620/event (plantlet)
$792/event (seed)

References:
  1. Armstrong CL, Green CE, Phillips RL (1991) Development and availability of germplasm with high Type II culture formation response. Maize Genetics Cooperative Newsletter 65: 92-93.
  2. Hallauer R, Lamkey KR, White PR (1997) Registration of five inbred lines of maize: B102, B104, B104, B105, and B106. Crop Sci 37: 1405–1406.
  3. Liu K, Goodman M, Muse S, Smith JS, Buckler E, Doebley J (2003) Genetic structure and diversity among maize inbred lines as inferred from DNA microsatellites. Genetics 165: 2117-2128.

Rice:
Our standard pipeline genotype is Japonica cv. Nipponbare for which genomic sequence information is available. We also can transform Japonica cv. Kitaake. Kitaake is a light insensitive variety, which may be desired by some researchers. However, our transformation frequency for Kitaake is lower than that of Nipponbare.

Nipponbare Kitaake
Photo period Light sensitive
(12 day/12 night)		 Light insensitive
(16 d/8 n, or 12 d/12 n)
Cost* $73/event (plantlets)
$113/event (seed) 		$145/event (plantlets)
$227/event (seed)
*our prices are based on minimum orders of 10 Nipponbare or 5 Kitaake events.

Soybean:
We can transform a number of public cultivars such as William 82, Williams and Thorne. William 82 is the cultivar with public genomic sequencing information.

Brachypodium:
Our standard line of Brachypodium distachyon is BD21.