IOWA STATE UNIVERSITY | DEPARTMENT OF AGRONOMY | COLLEGE OF AGRICULTURE AND LIFE SCIENCES | PLANT SCIENCES INSTITUTE

Choose a vector

b. Vectors for Agrobacterium-mediated method


The vector/Agrobacterium strain combination that have been performing consistently in our facility is pPZP derived vector1 and EHA1012. We strongly recommend to use this vector/strain combining with the bar gene/bialaphos selection for maize and soybean services in our facility. For rice and Brachypodium, both bialaphos and hygromycin can be used for selection. One recommended binary vector is pTF101.13 (see below for detail)

We can also perform transformation services using other vector/strain/selection systems upon customer request. However, such proposal needs to be thoroughly discussed with the facility (contact kanwang@iastate.edu) as a non-service collaboration and no transgenic product can be guaranteed.

References:
  1. Hajdukiewicz P, Svab Z, Maliga P (1994) The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol 25: 989-994.
  2. Hood EE, Helmer GL, Fraley RT, Chilton MD (1986) The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J Bacteriol 168: 1291-1301.
  3. Paz M, Shou H, Guo Z, Zhang Z, Banerjee A, Wang K (2004) Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant. Euphytica, 136: 167-179.