IOWA STATE UNIVERSITY | DEPARTMENT OF AGRONOMY | COLLEGE OF AGRICULTURE AND LIFE SCIENCES | PLANT SCIENCES INSTITUTE

Choose a vector

c. Request PTF Vectors

We can provide binary vector pTF101.1 for subcloning of your gene of interest for plant transformation. Note that the vector is not an expression vector, therefore, there is no promoter for your gene of interest. pTF101.1 is a suitable for you to subclone your entire gene cassette (promoter+gene+terminator).
The preferred Agrobacterium strain for pTF101.1 is EHA101 (a strain harbors a derivative of the agropine/L,L-succinamopine-type Ti-plasmid pTiBo542).
Basic features of the vector:
1. A broad host range (pVS1) origin of replication.
2. An aadA gene for bacterial selection of spectinomycin resistance.
3. The T-DNA right and left border fragments from nopaline strain of A. tumefaciens.
4. A selectable marker gene cassette containing (1) 2x CaMV 35S promoter, (2) TEV (Tobacco Etch Virus translational enhancer), (3) bar coding region, (4) nos terminator.
5. A multiple cloning site containing seven unique restriction enzyme sites (Hind III, Xba I, BamH I, Xma I, Sma I, Sac I, EcoR I).
Types of pTF101.1
1. MCS version (map, components, sequences, publication, MTA)
  pTF101.1 contains a multiple cloning site carrying seven unique restriction enzyme sites (Hind III, Xba I, BamH I, Xma I, Sma I, Sac I, EcoR I).
2. Gateway version
  1. pTF101.1gw1 (map, components, MTAgw) This Gateway one-entry version contains attR1 and attR2 recombination system suitable for subcloning your Gateway compatible gene of interest cassette (promoter+gene+terminator).
  2. pTF101.1gw3 (map, components, MTAgw) This Gateway three-entry version contains attR3 and attR4 recombination system suitable for subcloning your Gateway compatible promoter, gene and terminator fragments all at once.
Please follow the steps below for requesting these vectors:
1. Down load the standard Material Transfer Agreement (MTA, MTAgw).
2. Complete MTA by filling the information and obtaining signatures of both Principle Investigator (PI) and the institutional authorized officer.
3. Send your request by e-mail to Kan Wang (kanwang@iastate.edu) including full mailing address and specific request on desired vector.
4. Send back the signed MTA by fax 515-294-2299 (attention Kan Wang) or electronic attachment to kanwang@iastate.edu.
5. Send an acknowledgement mail to kanwang@iastate.edu upon receiving the vector DNA by mail.