IOWA STATE UNIVERSITY | DEPARTMENT OF AGRONOMY | COLLEGE OF AGRICULTURE AND LIFE SCIENCES | PLANT SCIENCES INSTITUTE

Soybean Genetic Transformation:
Agrobacterium-mediated

Bialaphos resistant soybean plantlet on regeneration medium

The PTF Soybean Transformation System:

Method optimized in PTF lab (Paz et al. 2004)
Target tissue: Half-seed explants
Transformable cultivars: Thorne, Williams, Williams 82
Delivery means: Agrobacterium tumefaciens
Selection: the bar gene/bialaphos
Products available: seed
Protocol: please see Soybean Transformation Protocol

Transformation efficiency:

3%

Number of transgenic events confirmed in R1 per infected explants

To order your transgenic soybean:

  1. Obtain APHIS permit
  2. Request Agrobacterium vector
  3. Sumbit your construct
  4. Receive transgenic R1 seeds


Time frame from infection to transgenic seeds:

Transformation Activity Day
Explant dissection and infection 1
Selection and shoot regeneration of bialaphos resistant events 6 (8-12 wks)
Rooting 56
Plants to soil 70
Harvest 240
Progeny analysis and delivery 270