IOWA STATE UNIVERSITY | DEPARTMENT OF AGRONOMY | COLLEGE OF AGRICULTURE AND LIFE SCIENCES | PLANT SCIENCES INSTITUTE

Brachypodium distachyon Genetic Transformation:
Agrobacterium-mediated

Immature seeds are used as the starting material for Brachypodium transformation (top). Plantlet regeneration on tissue culture medium (bottom).

The PTF Brachypodium Transformation System:

Target tissue: Callus culture derived from immature embryo
Transformable cultivars: BD-21 Delivery means: Agrobacterium tumefaciens
Selection: the bar gene/bialaphos or the hpt gene/hygromycin
Products available: Plantlets

Transformation frequency:

18%

Transformation frequency is defined as the number of bialaphos resistant callus per 100 infected callus pieces.

To order your transgenic Brachy:

  1. Obtain APHIS permit
  2. Request Agrobacterium vector
  3. Submit your construct
  4. Receive transgenic plantlets


Time frame from callus initiation to transgenic plantlets:

Transformation Activity Day
Initiate callus from immature embryo 1
Select callus for transformation 22
Transform callus tissue 26
Pick and bulk bialaphos resistant callus events 54-68
Regenerate transgenic plantlets 82-96