Maize Genetic Transformation:
Biolistic-mediated
The PTF Maize Transformation System:
Method optimized in PTF lab (Frame et al. 2000)
Target tissue: Immature zygotic embryos or callus cultures derived from immature embryo.
Transformable genotypes: Hi Type II hybrid;
Delivery means: BioRad PDS 1000/He biolistic gun
Selection: the bar gene/bialaphos
Products available: Callus, plantlets, seed
Protocol: please see Maize Embryo Bombardment Protocol or Maize Callus Bombardment Protocol
Maize immature embryo bombarded with CaMV35S driven GFP. Picture taken 48h after bombardment
Time frame from bombardment to transgenic plant/seed:
| Transformation Activity | Day |
|---|---|
| Embryo bombardment | 1 |
| Pick and bulk bialaphos resistant callus events | 60-90 |
| Confirm co-transformation | 90-110 |
| Regenerate transgenic plantlets to soil | 120-150 |
| Harvest transgenic seed in greenhouse | 230-260 |
