IOWA STATE UNIVERSITY | DEPARTMENT OF AGRONOMY | COLLEGE OF AGRICULTURE AND LIFE SCIENCES | PLANT SCIENCES INSTITUTE

Maize Genetic Transformation:
Biolistic-mediated

Maize immature embryo bombarded with CaMV35S driven GFP. Picture taken 48h after bombardment

The PTF Maize Transformation System:

Method optimized in PTF lab (Frame et al. 2000)
Target tissue: Immature zygotic embryos or callus cultures derived from immature embryo.
Transformable genotypes: Hi Type II hybrid;
Delivery means: BioRad PDS 1000/He biolistic gun
Selection: the bar gene/bialaphos
Products available: Callus, plantlets, seed
Protocol: please see Maize Embryo Bombardment Protocol or Maize Callus Bombardment Protocol

Transformation frequency:

15%

Transformation frequency is defined as the number of bialaphos resistant callus per 100 bombarded embryos.

To order your transgenic maize:

  1. Submit your construct
  2. Obtain APHIS permit or notification
  3. Establish analysis system and receive callus samples
  4. Receive transgenic plantlets
    OR
    Receive transgenic seeds

Time frame from bombardment to transgenic plant/seed:

Transformation Activity Day
Embryo bombardment 1
Pick and bulk bialaphos resistant callus events 60-90
Confirm co-transformation 90-110
Regenerate transgenic plantlets to soil 120-150
Harvest transgenic seed in greenhouse 230-260