1. Submit 2. APHIS 3. Analysis 4. Plantlets or Seed
Receive transgenic plantlets
You will be notified by e-mail 1-2 weeks before plantlets are ready to be sent. The list below will give you an idea of the timing. You may receive shipments of plantlets over several different dates because plantlets develop at different rates.
Maize Biolistic: 5 weeks after we receive your molecular confirmation data
Maize Agro: 14 weeks after Agro infection
Rice: 3.5 months after Agro infection
Maize
If you intend to grow transgenic maize plants to maturity in your own greenhouse, keep in mind that you will need to plan for planting tester lines as well. These tester lines will serve as your pollen donor plants for transgenic silks, or silk recipient plant for transgenic pollen, since it is usually difficult to have a transgenic plant to produce its silks and tassel at the same time.
You will need to start planting 2-3 donor/recipient plants every 3 days, beginning when you receive an e-mail from us indicating your plantlets are almost ready and finishing after notification from PTF that the last shipment of plantlets was sent. This will ensure you a good flow of pollen/silk for your transgenic material.
Maize greenhouse protocol
Rice
The cultivar of rice we transform (Nipponbare) requires a 12:12 light/dark cycle. If your region of the country does not provide this, you must use a growth chamber to grow your plants. Transgenic rice plantlets you receive from us will not set seed if grown at a daylength longer than 12 hours.
