BREAD - Genome Enabled Platforms for Yam

Yam is a major source of dietary starch for smallholder farm families in many tropical and subtropical countries. Despite its global importance, use of modern genomics tools that can facilitate our fundamental understanding of how to improve the productivity and nutritional quality of the crop has lagged behind that of other major crops. The overall goal of this proposed project, therefore, is to establish genomeenabled platforms for yam. When successfully completed, the resources generated in this project, including facile sitedirected genome editing (e.g., CRISPR/Cas9 system) and robust genetic engineering methods, will provide effective tools for the fundamental understanding and genetic improvement of this important crop. Specific objectives include:

1. Establishing efficient CRISPR/Cas9 based site-directed mutagenesis systems for yam
2. Producing and characterizing CRISPR/Cas9mutagenized yam plants
3. Conducting a focused workshop in IITAKenya to share the latest transformation developments and genome editing protocols with African scientists
4. Providing shortterm laboratory training experience for African woman scientists
5. Leading a two-week study abroad course in cooperation with IITATanzania to provide Iowa State

04/01/2016 to 03/31/2019
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